It is known that the activity of some of the same genes is essential for germ cell differentiation and the maintenance of pluripotency. Here, we chemically induced a cellular state with some molecular markers of male gonocyte induction in male mouse ES cells in vitro, which we termed gonogenic stimulated transition (GoST). Interestingly, the resulting GoST cells differentiated without further stimulation into cells with several markers typical for early gonocytes, but when stimulated, also showed an enhanced multilineage differentiation potential compared to ES cells in vitro. This work establishes the basis to generate germ cells for diverse applications in research and medicine.
Moshfegh, C. et al. A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways. Scientific Reports. doi: 10.1038/srep25104